Development of cecal predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis.

BLAJMAN, J.E.; ZBRUN, M. V.; SIGNORINI, M.L.; ZIMMERMANN, J.A.; ROSSLER E.; BERISVIL, A.P.; ROMERO SCHARPEN A.; ASTESANA D.M.; SOTO, L.P.; FRIZZO, L.S.

ANIMAL PRODUCTION SCIENCE

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2016

1836-5787

Understanding of the intestinal microbiota is crucial to enhance intestinal health and performance parametersin animals. A more exhaustive research of the intestinal microbiota of broilers could be of interest to implementappropriate intervention measures. The aim of the present study was to investigate the development of the predominantcecal microbiota in broilers that were fed a Lactobacillus salivarius DSPV 001P strain during a complete rearing usingdenaturing gradient gel electrophoresis (DGGE). Bacterial DNA from cecal samples of 24 broilers at different ages wereamplified by PCR and analysed by DGGE. A total of 35 DGGE products were excised and sequenced. Distinctivedifferences in bacterial communities were observed in the caecum as broilers age. At early stages, identified bacteria withinthe caecum of broilers were predominantly Clostridium-related species. Also, some sequences had the closest match to thegenus Escherichia/Shigella. Furthermore, the caecum was a reservoir rich in uncultured bacteria. The major differenceobserved in our study was an increase of potentially beneficial Lactobacillus at Day 45. These results may be attributedto modulation of the microbiota by the probiotic supplementation. The obtained data could be relevant for future studiesrelated to the influence of the microbiota resulting from probiotic supplementation on the performance and theimmunological parameters of broilers.