INVESTIGADORES
OMARINI Alejandra Beatriz
artículos
Título:
Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone
Autor/es:
OMARINI ALEJANDRA; PLAGEMANN INA ; SCHIMANSKI SILKE; KRINGS ULRICH ; BERGER RALF G.
Revista:
BIORESOURCE TECHNOLOGY
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 171 p. 113 - 113
ISSN:
0960-8524
Resumen:
Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow x slow and the fast x fast dikaryotic crosses showed similar or inferior yields. Some slow x fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60 %) and lipoxygenase activity of the strains.
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