INVESTIGADORES
MAZZAFERRO Laura
artículos
Título:
Dose-dependent significance of monosaccharides on intracellular a-L-rhamnosidase activity from Pseudoalteromonas sp. 005NJ.
Autor/es:
MAZZAFERRO, LAURA; ORRILLO, GASTON A.; LEDESMA, PABLO; BRECCIA, JAVIER D.
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Año: 2008 vol. 30 p. 2147 - 2150
ISSN:
0141-5492
Resumen:
Intracellular a-L-rhamnosidase (EC 3.2.1.40) from the psychrotolerant Pseudoalteromonassp. 005NJ showed a dose-dependent inhibition for L-rhamnose (IC50 = 20 mM) and D-ribose (IC50 = 95 mM), whereas D-glucose and L-fucose presented a lower inhibition, with IC50 values as high as >0.5 and >0.2 M, respectively. On the other hand, D-fructose enhanced enzyme activity threefold, reaching a plateau of maximum specific activity between 0.2 and 0.4 M of this monosaccharide. Both effects, low inhibition and stimulation, caused by key fruit sugars (glucose and fructose), make this biocatalyst an interesting system in terms of its potential application for debittering fruit juices.
