CONICET | Buscador de Institutos y Recursos Humanos

Since last years, antibodies are considered one of the most important biotechnological products. They have a wide application in human therapy, diagnostics and research. Pichia pastoris had shown to be one of the main production systems, due to its high cellular density, low extracellular proteases concentration and correct heterologous proteins folding. This work evaluates the production of the antibody scFv anti LDL from Pichia pastoris by using different nitrogen sources to optimize the cost/benefit of the process. The yeast culture was accomplished in a 200 ml shaker using BMGY media. After 28 hours of growth at 30°C, the production of antibody was induced with methanol at 1% P/P during periods of 12 hours until a final cultivation time of 96 hours. The nitrogen sources used were wheat flour, rice flour and casein peptone. Biomass at different times and the final antibody concentration were determined. Biomass was evaluated at different times and the antibody concentration was determined in the end of the process. By comparing the different nitrogen sources it could be observed that the casein peptone medium resulted in the highest cellular growth and antibody production. The casein peptone point out to be four times superior in antibody production comparing with the wheat flour and three times better than the production obtained with the rice flour. Nevertheless, the costs analysis for the production of 1g of the antibody for each nitrogen source, considering their actual price, indicates that these alternative substrates are a better choice than the casein peptone. The wheat flour resulted to be 18 times cheaper than the casein peptone while the rice flour cost was 118 times lower. Rice flour showed the best cost-benefit in antibody production.

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