Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition

ELISA C. ALE; VIRGINIA BATISTELA; GABRIELA CORREA OLIVAR; JOANA B. FERRADO; SOHAIB SADIQ; HAFIZ AHMED; JORGE A. REINHEIMER; LUCIANA VERA CANDIOTI; ANDREW P. LAWS; ANA G. BINETTI

INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2020

1364-727X

Lactobacillus fermentum Lf2 produces high amounts of exopolysaccharides (EPS) (~1 g/L) with demonstrated functional and technological roles when applied as a food ingredient in dairy matrices, properties that made these EPS interesting in comparison with other similar molecules from lactic acid bacteria (LAB). Those characteristics encouraged us to optimise the production. The EPS extract is composed of a high molecular mass β-glucan and a medium molecular mass heteroglycan. In the present work, the optimal conditions that doubled the EPS yield using a semi-defined medium (SDM, 0.63% yeast nitrogen base, 0.53% bacto casitone, 0.53% ammonium citrate, 6.25% sucrose, pH 6.5) were found by means of response surface methodology (RSM). The chemical characterization indicated that under optimised conditions the synthesis of the heteroglycan was favoured compared with that of the β-glucan.Keywords: exopolysaccharides; statistical optimisation; chemical composition, L. fermentum; response surface methodology.