Lactobacillus plantarum CIDCA 83114 reduces the pathogenic potential of Shiga toxin (stx)-producing Escherichia coli (STEC) O157:H7.

FERNANDEZ BRANDO RJ; CARBALLO D; BERNAL A; SOSA FN; RAMOS MV; ABRAHAM A G; GARROTE G,L; PALERMO M S

Congreso; LXX REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) & 3ER CONGRESO FRANCO-ARGENTINO DE INMUNOLOGÍA (FAIC); 2022

Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 is a foodborne pathogen, which can lead to the life-threatening Hemolytic Uremic Syndrome (HUS). There is no treatment available in order to reduce HUS outcome up to date. Probiotics are live microorganisms that promote beneficial health effects on the host. Lactobacillus plantarum CIDCA 83114 (CIDCA 83114) was previously isolated from kefir and reduced the cytotoxic activity of Stx on Vero cells. The aim of this work was to evaluate the effect of CIDCA 83114 on the pathogenic potential of a clinically isolated STEC strain. We evaluated the inhibition of STEC growth by CIDCA 83114 by co-culturing both bacterial strains in ratios 1:1, 1:10 and 1:100 (STEC: CIDCA 83114) and counting colony forming units by plating in specific media. Besides, we also tested the competition and exclusion exerted by CIDCA 83114 on STEC adhesion to Caco-2 cells. Results were statistically analyzed by Kruskal-Wallis test.We observed that CIDCA 83114 completely inhibited STEC growth by 24 h co-culture in all the ratios tested (p