Probiotic yeast Kluyveromyces marxianus for prevention of low-grade inflammation derived tumorigenesis: proof of concept in a murine model of colorectal cancer.

FERREYRA M; CUCCA G; PENDON M.D.; GARROTE G L; ROMANIN D. E.; RUMBO M

Buenos Aires

Congreso; LXX REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) & 3ER CONGRESO FRANCO-ARGENTINO DE INMUNOLOGÍA (FAIC); 2022

Colorectal cancer (CRC) is the third most prevalent cancer worldwide. It is known that chronic inflammation increases the risk of tumor development. Our group has demonstrated that Kluyveromyces marxianus CIDCA 8154 strain (Km 8154) has immunomodulatory properties which could modify the context for tumor development. Therefore, here we evaluate its administration to impact the process of tumorigenesis in a murine model of colitis-associated colorectal cancer by administration of azoxymethane/dextran sodium sulfate (AOM/DSS). After intraperitoneal injection of AOM 10 mg/kg, the AOM/DSS group were provided with 2,5% DSS in drinking water for 5 consecutive days, followed by 16 days of regular drinking water. This cycle was repeated 4 times. Mice were administered 10mL of a yeast suspension on gelatin (1-3 108 UFC/cage) every 48 hours, control mice received gelatin without yeast. Mice were monitored and weighted daily and euthanized at week 13. General inflammation markers, such as colon length and liver weight, were measured. No difference in these markers nor in the weight fluctuation between the AOM/DSS groups with or without Km 8154 were found. We counted the presence of polyps in the colon to discover that there are fewer polyps in the group AOM/DSS+Km 8154 compared to the control AOM/DSS group (p