Immunochemical methods for detection of probiotics

ABRAHAM A G; SERRADELL M A; GARROTE G L; FOSSATI A C; DE ANTONI G L

Probiotics in food safety and human health

Taylor and Francis, CRC Press

Lugar: Inglaterra; Año: 2005; p. 165 - 189

CONTENTS: Antigenic Molecules and Structures Associated with Probiotic Characteristics. Application of Immunochemical Techniques to Potentially Detect Probiotic Bacteria. Methodology for Immunochemical Detection of Probiotics. Preparation of Antigens. Preparation of Bacterial Antigens. Purification of Bacterial Proteins. Production of Specific Antibodies. Production of Polyclonal Antibodies (Antisera). Preparation and Production of Monoclonal Antibodies.Labeling of Antibodies. Labeling Antibodies with fluorochromes. Labeling Antibodies with Biotin. Labeling Antibodies with Enzymes. Methods for Detection. Indirect Immunofluorescence. Noncompetitive Indirect ELISA. Competitive Sequential ELISA. Capture ELISA. Immunoblotting or Western Blot. Dot Blot.Flow Cytometry. Conclusions.