Covalent attachment of Aspergillus oryzae lipase on chemically modified hybrid matrixes

MENDES, ADRIANO; RODRIGUES, DASCIANA; ADRIANO, WELLINGTON; MAMMARELLA, ENRIQUE; CASTRO, HEIZIR; GIORDANO, RAQUEL

Montpellier, Francia

Conferencia; 10th Internacional Conference on Chitin and Chitosan; 2006

European Chitin Society

This work aims to obtain thermo-stable immobilized derivatives on hybrid matrixes consisted of chitosan and different copolymers, as k-carrageenan, gelatin or alginate. Lipase from Aspergillus oryzae was immobilized by multipoint covalent attachment on chitosan-copolymer and pure chitosan (as control). The hybrid matrixes were chemically modified with 2,4,6 trinitrobenzene sulphonic acid (TNBS) to increase the support hydrophobicity followed by activation with Glycidol (GL), Epichlorohydrin (EP) or Glutaraldehyde (GA). Hybrid matrixes that provided immobilized derivatives with high hydrolytic activity were chitosan-alginate-TNBS activated with glycidol (281.5 U/g of gel), glutaraldehyde (231.5 U/g of gel), chitosan-alginate without TNBS activated with epichlorohydrin (308.7 U/g of gel) and chitosan-glycidol cross-linked with GA 1% (249.7 U/g of gel). For all immobilized derivatives, a 45-fold increase in the thermal stability was observed in relation to the free lipase.