INVESTIGADORES
MANZO Ricardo Martin
artículos
Título:
Purification of two bacteriocins produced by Enterococcus faecalis DBFIQ E24 strain isolated from raw bovine milk
Autor/es:
MANZO, RICARDO M.; CARDOSO, MARÍA DE LAS MERCEDES; TONARELLI, GEORGINA G.; SIMONETTA, ARTURO C.
Revista:
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2016 vol. 69 p. 282 - 293
ISSN:
1364-727X
Resumen:
Enterococcus faecalis DBFIQ E24 was formerly characterised as a producer of antibacterial and antifungal substances. This strain is vancomycin sensitive, nonhaemolytic and gelatinase negative. SDS-PAGE revealed the presence of an active band located between 1060 and 3500 Da. After the application of a three-step purification procedure, two antimicrobial peptides were isolated. One of them is mostly active against Bacillus cereus with a molecular mass of 1364 Da, and the other one with a molecular mass of 1686 Da is mainly active against Escherichia coli. These results confirm the technological potential of E. faecalis DBFIQ E24 strain as a GRAS food biopreservative.
