Development and evaluation of ssr markers for garlic and onion

BARBOZA ROJAS, K.; GALMARINI, C.R.; CAVAGNARO, P. F.

Mendoza

Otro; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2016

Sociedad de Biología de Cuyo

The genus Allium includes economically important vegetables, consumed worldwide due to their characteristic flavor. Globally, garlic and onion are the most important Allium. INTA La Consulta is home of breeding programs for both vegetables. Despite its importance, the molecular tools available for Allium are very limited. In the present study we developed and evaluated 261 microsatellite markers (or SSRs) from genetic and genomic level of polymorphism in four genetically different cultivars of garlic and onions. Analysis of the data revealed that 31 (12%) of these markers were polymorphic in onion cultivars and 44 (17%) were in garlic. Also a significant number of garlic and onion SSRs were successfully transferable across Alliaceae. 75 (EST and genomic SSRs) and 51 (ESTSSRs) primer pairs that produced fragments inGarlic and onion respectively, also produced putative SSR-containing products in one or more Alliaceae species. The SSRs markers developed in this study will impact positively the garlic and onion national breeding programs.