Genetic diversity of Phytophthora capsici isolates from pepper and pumpkin in Argentina

GOBENA, D. ; ROIG, J. M.; GALMARINI, C.R.; HULVEY, J.; LAMOUR K

MYCOLOGIA.

ALLEN PRESS INC

Lugar: Lawrence; Año: 2011 vol. 2011 p. 14711 - 14711

0027-5514

 Phytophthora capsici is a soil-born oomycete plant pathogen that limits pepper  production worldwide. The population structure varies significantly depending on the location(e.g. Peru vs. the USA) and very little is known about the diversity of P. capsici in Argentina. Our objective was to assess the diversity of P. capsici in Argentina at key pepper production areas. From 2006 to 2009, 40 isolates were recovered from pepper and one isolate from pumpkin at eleven locations. Isolates were assessed for mating type, mefenoxam sensitivity, and multilocus single nucleotide polymorphism (SNP) genotype profiles. Ten isolates with identical SNP profiles were also genotyped using amplified fragment length polymorphism (AFLP) markers. All 41 isolates had the A1 mating type and were sensitive to mefenoxam. Genotypic analysis using eight polymorphic SNP markers indicated 87% of the isolates had the same multi-locus genotype which is fixed for heterozygosity at 7 of the 8 SNP sites. AFLP analyses confirmed these findings, and overall, it appears that clonal reproduction drives the population structure of P. capsici in Argentina. The implications for breeding resistant peppers and overall disease management are discussed.