Saccharomyces cerevisiae straisn from animal environment with aflatoxin B1 binding abilitily and anti-pathogenic bacterial influence.

ARMANDO M.R.; PIZZOLITO R.P.; ESCOBAR F.; DOGI C.A.; PEIRANO M.S.; SALVANO M.A.; SABINI L.I.; COMBINA M.; DALCERO A.M.; CAVAGLIERI M.L.

World Mycotoxin Journal

Wageningen Academic Press

Lugar: Bilthoben, The Netherland; Año: 2011 vol. 4 p. 59 - 68

1875-0710

Mycotoxins are metabolites produced by fungi growing on foods and representa serious hazard to humans and animal. Concerns related to hre negative health impact of aflatoxins hace led to the investigation of strategies to prevent, eliminate or reduce the presence of these toxins in contaminated products. Saccharomyces cerevisiae strains are among promisin candidates that can be used in animal feed for improving the robustness of animals in the production environment. The aim of this work was to isolate and select S. cerevisiae strains from pig environment with aflatoxin B1 (AFB1) binding ability, able to tolerate gastrointestinal conditions and with some potential beneficial properties to the host.S. cerevisiae strains were isolated form animal feed, faeces and gutand identified by morphological and molecular techniques. AFB1 binding percentages varied among the yeast strains according to the AFB1 concentration used. The RC016 strain showed the highest adsortion percentage at the three AFB1 concnetrations tested in this work (50, 100 and 500 ng/mL) followed by EC008 strain. All yeast strain were able to survive under gastrointestinal conditions and to strongly adhere to Vero cells. All S. cerevisiae strains showed co-aggregation with pathogenic bacteria (Escherichia coli, Enterobacter cloacae and Salmonela enterica sub sp. enterica). Only RC016 and RC008 strongly inhibited the three pathogens assayed. S. cerevisiae strain RC016 and RC008 are promising microorganisms for inclusion in animal feed.