Whey protein coating bead improves the survival of the probiotic Lactobacillus rhamnosus CRL 1505 to low pH

GEREZ C.L., ; FONT DE VALDEZ G., ; GIGANTE M.L.,; GROSSO C.R.F..

LETTERS IN APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2012 p. 552 - 556

0266-8254

Aims: To evaluate the efficacy of a novel microencapsulation procedure using whey protein and pectin to improve the survival rate of Lactobacillus rhamnosus CRL 1505 to low pH and bile. Methods and Results: Lactobacillus rhamnosus CRL 1505 was encapsulated by ionotropic gelation using pectin (PE) and pectin/whey protein (PE-WP). Both types of beads (MC PE-WP and MC PEWP-WP) were covered with a layer of whey protein by complex coacervation. The noncapsulated lactobacilli were not sensitive to bile salts but to acid. Both microparticles protected Lact. rhamnosus CRL 1505 at pH 2.0, but only MC PE-WP was effective at pH 1,2. Conclusions: The combination of ionotropic gelation and complex coacerva- tion techniques is efficient to obtain microcapsules of pectin covered with whey proteins. The MC PE-WP beads were more stable than the MC PEwP-WP beads insimulated gastric conditions, thus offering better protection to Lact. rhamnosus CRL 1505 at low pH. Significance and Impact of the Study: Pectin beads with a whey protein layer (MC PE-WP) could be used as probiotic carrier in functional foods of low pH (e.g. apple juice), thus protecting Lact. rhamnosus CRL 1505 against the stressful conditions of the gastric tract