INVESTIGADORES
FADDA Silvina Graciela
congresos y reuniones científicas
Título:
Salt Tolerance of Lactobacillus sakei for the Design of New Salted Anchovy-Based Products
Autor/es:
1.VIGNOLO G; BELFIORE, C.; FADDA S,
Lugar:
Copenhague
Reunión:
Simposio; 22nd International ICFMH Symposium Food Micro 2010; 2010
Institución organizadora:
ICFMH
Resumen:
Anchovy (Engraulis anchoita) is considered one of the most abundant pelagic fish
species from South West Atlantic Ocean. Anchovy is processed for human consumption
only in Argentina coming from the so-c alled anchoita Bonaerense stock; the diversified
production targets mainly the external market being a leading producer of salted
anchovy-based products. Prevalence of different lactic acid bacteria (LAB) genera in fish
is a direct consequence of the environmental c onditions existent before and af ter
capture. Although some lactic acid bacteria (LAB) species have become a major source
of concern in aquaculture due to its pathological significance, LAB are able to grow on
fish subjected to different technological processes. In previous studies, Leuconostoc and
Lactobacillus were identified and characterized as the m ain genera present in f resh
anchovies. Technological and safety traits of these strains showed Lactobacillus sakei
SACB7 04 to be unable to produce dextran and biogenic amines, exhibiting sensitivity to
chloranfenicol, erythromycin and tetracycline and containing gdh gene. Considering that
salt can be a barrier to be overcome by LAB in new anchovy -based products, resistance
of this isolate t o N aCl was evaluated in anchovies m uscle extract. L. sakei SACB704
showed NaCl tolerance surviving during three months in presence of 10% of NaCl and
f ifteen days in extracts containing 15-18% NaCl. When the addition of osmoprotectant
compounds was studied, improved growth of L. sakei was found indicating they were
protective under salt-induced stress. To ev aluate L. sakei SAC B7 04 ability to wit hstand
adverse environmental stress in presence and absence of glycine betaine a proteomic
approach was applied