EFFECT OF LACTOBACILLUS FERMENTUM CRL1446 ON IMMUNE-METABOLIC PARAMETERS AND INTESTINAL MICROBIOTA COMPOSITION IN OBESITY IN HIGH-FAT DIET FED MICE

GAUFFIN CANO, MARIA PAOLA; FABERSANI, EMANUEL; MARQUEZ, ANTONELA; ROSS, ROMINA; ABEIJON MUKDSI, MARIA CLAUDIA; MEDINA, ROXANA BEATRIZ

Buenos Aires

Congreso; 21st International Congress of Nutrition; 2017

Sociedad Argentina de Nutrición; International Union of Nutritional Sciences (IUNS)

Background and objectives: Dietary strategies, including the use of probiotics as preventive agents that handle the intestinal microbiota and/or modulate the function of adipose tissue, would be suitable tools for the prevention or treatment of different conditions associated with obesity. In this context, our aim was to evaluate the effect of Lactobacillus fermentum CRL1446 (CRL1446) on metabolic and immunological parameters and on the composition of intestinal microbiota in a model of obesity induced by a high-fat diet (HFD). Methods: Balb/c mice were separated into the following groups: 1) SD: Standard diet, 2) HFD: High-fat diet, 3) HFD+CRL1446: mice receiving a HFD and a daily dose of 1 x 108 CFU of L. fermentum CRL1446 (CRL1446) in the drinking water. The dietary intervention was maintained for 6 weeks. Weight and food intake were monitored daily. It were determined at 20 and 45 days of experimental period: fat tissue weight (histologic studies), metabolic parameters (triglyceride (TG), total cholesterol (TC), glucose (Glu) and leptin (Lep)), seric immunologic parameters (TNF-alpha, IL-6, IL10 and MCP-1), macrophage and adipocyte functionality (ex vivo studies) and the gut microbiota composition (evaluated by high-throughput sequencing of 16S rRNA gene amplicons). Results: CRL1446 strain induces a decrease in body and adipose tissue weights and the size of adipocytes. A decrease in plasmatic glucose, cholesterol, triglycerides, leptin, TNF-alpha and IL-6 levels and an increase of IL-10 were observed. Ex vivo studies showed a minor secretion of leptin by adipocytes. In macrophages, CRL1446 induced lower levels of MCP-1, TNF-alpha, IL-6 and higher levels of IL-10. Regarding intestinal microbiota, an increase in Lactobacillus and a decrease in Firmicutes/Bacteroidetes (F/B) index were observed. Conclusions: The supplementation with L. fermentum CRL1446 produce changes in intestinal microbiota structure and metabolic and immunologic parameters. CRL1446 could potentially be a probiotic strain useful to correct the immuno-metabolic alterations associated with obesity through its adipomodulatory capacity.