Compatibility and safety of five lectin-binding putative probiotic strains.Protection against dietary lectins cytotoxicity on intestinal epithelial cells of broilers

BABOT J.D.; ARGAÑARAZ MARTÍNEZ E.; SAAVEDRA L.; APELLA M. C.; PEREZ CHAIA A

Beneficial Microbes

Wageningen Academic Publishers

Lugar: Wageningen; Año: 2018 vol. 9 p. 927 - 935

1876-2883

The banning of antibiotics used as feed additives for animal growth promotion in theEuropean Union and United States and the expectation of this trend to further expand toother countries in the short term have prompted a surge in probiotic research. Multispeciesprobiotics including safe and compatible strains with the ability to bind differentnutritional lectins with detrimental effects on poultry nutrition could replace antibioticsas feed additives. Lactobacillus salivarius LET201, L. reuteri LET210, Enterococcusfaecium LET301, Propionibacterium acidipropionici LET103 and Bifidobacteriuminfantis CRL1395 have proved to be compatible as evaluated through three differentapproaches: the production and excretion of antimicrobial compounds, growthinhibition by competition for essential nutrients and physical contact, and a combinationof both. The safety of P. acidipropionici LET103 was confirmed, since no expression ofvirulence factors or antibiotic resistance was detected. The innocuity of E. faeciumLET301 should be further evaluated since the presence of genes coding for certainvirulence factors (gelE, efaAfm and efaAfs) was observed, albeit no expression of gelEwas previously detected for this strain and there are no reports of involvement of efaAfmin animal pathogenicity. Finally, a combination of the five strains effectively protectedintestinal epithelial cells of broilers from the cytotoxicity of mixtures of soybeanagglutinin (SBA), wheat germ agglutinin (WGA) and Concanavalin A (Con A). To ourknowledge, this is the first time that a combination of strains is evaluated for theirprotection against lectins that might be simultaneously present in poultry feeds.