INVESTIGADORES
TARANTO Maria Pia
artículos
Título:
Relationship between bile salt hydrolase activity, changes in the internal pH and tolerance to bile acids in lactic acid bacteria
Autor/es:
BUSTOS, ANA YANINA; LUCILA SAAVEDRA; FONT DE VALDEZ, GRACIELA; RAYA, RAUL; TARANTO, MARIA PIA
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2012 vol. 35 p. 1511 - 1518
ISSN:
0141-5492
Resumen:
The effect of the conjugated bile acid (BA) on the microbial internal pH (pHin) values in lactic acid bacteria with and without ability to hydrolyze bile salts (BSH [+] and BSH [-] strains, respectively) was evaluated. BSH (+) strains showed a gradual increase in the pHin following the addition of conjugated BA; this behavior was more pronounced with GDCA than with TDCA may be due to the higher affinity of BSH for the glyco-conjugates acids. Conversely, the BSH (-) strains showed a decrease in internal pH probably as a consequence of weak acid accumulation. As it was expected, a decrease in the cytoplasmatic pH affected the cell survival in this last group of strains, while the BSH (+) strains were more resistant to the toxic effect of BA.
