Protective effect of Lactiplantibacillus plantarum LP5 in a murine model of colonization by Campylobacter coli DSPV458

RUIZ, MARÍA JULIA; SIRINI, NOELÍ; SIGNORINI, MARCELO; ETCHEVERRÍA ANALÍA; ZBRUN, VIRGINIA; SOTO, LORENA; ZIMMERMANN, JORGE; FRIZZO, LAUREANO

Beneficial Microbes

Wageningen Academic Publishers

Lugar: Wageningen; Año: 2021 vol. 0 p. 1 - 14

1876-2883

Thermotolerant Campylobacter species are the leading cause of foodborne bacterial diarrheal disease worldwide. Campylobacter coli, abundant in pigs and pork products, have been identified as a source of human infection. In this study, we propose the use of Lactiplantibacillus plantarum LP5 as a probiotic to reduce colonization of this intestinal pathogen in a murine colonization model of C. coli DSPV458. Six-week-old adult female Balb/cCmedc mice were housed in groups: Control, Campy and Pro-Campy. Control and Pro-Campy groups received antibiotics for 5d and the Campy group for 12d. Pro-Campy group was inoculated for 7d with 8.78log10CFU total of L. plantarum LP5 suspended in MRS. All groups were inoculated with 6.72log10CFU of C. coli DSPV458 suspended in BHI. L. plantarum LP5 was recovered only in Pro-Campy group. C. coli DSPV458 was recovered at higher levels in Control and Campy groups. The differences with Pro-Campy group were significant. As regards feces, Control and Campy groups reached 7.41 and 7.84 log10CFU/g respectively, and Pro-Campy group only 4.62log10CFU/g. In the cecum, Control and Campy groups reached 8.01 and 9.26 log10CFU/g, respectively, and Pro-Campy group only 4.51 log10CFU/g. In the ileum, Control and Campy groups reached 3.43 and 3.26 log10CFU/g, respectively, and Pro-Campy group did not show detectable levels. The reduction of C. coli DSPV458 in Pro-Campy group compared to Control group in feces, cecum and ileum was 99.55%, 99.98% and 100%, respectively. Animals were maintained under normal health conditions, and hematological parameters were within the standard values for Balb/cCmedc. The incorporation of a probiotic generated a protective effect in the mice colonization model. The protective effect would also apply to intestinal colonization by indigenous enterobacteria. Therefore, the strategy used in this study is of great importance to understand the protection mechanisms in a murine model, as well as its application in food-producing animals.