Evaluation of nutraceutical and antioxidant properties of andean landraces tomatoes

DI PAOLA NARANJO R.D.; SARAGUSTI A.; OTAIZA S.N.; VALLE, E.M.; PERALTA, I.; CARRARI, F.; ASIS RAMÓN

Buenos Aires

Congreso; VI International Conference on Polyphenols and Health; 2013

UBA

Tomato is one of the most important sources of nutraceutical compounds to the western diet. In this work, we assessed the antioxidant metabolites composition in mature tomatoes from Argentine Andean Valleys and their biological activity, in order to identify the active components responsible of their nutraceutical properties. Antioxidant metabolites from tomato hydrophilic extracts were determined by HPLC-DAD-MS/MS. In vitro antioxidant capacity (AC) was determined by TEAC and FRAP methods. Biological activity was evaluated using a Caenorhabditis elegans model exposed to thermal stress. Two out of seventeen cultivars showed the significant highest AC measured by in vitro methods, however, five out of seventeen cultivars showed the significant highest biological activity. The contribution of each phenolic compound to the antioxidant activity and biological properties was evaluated statistically by a multiple regression model. Chlorogenic acid was the compound with the highest positive contribution to the biological activity, which was after confirmed by C. elegans assay, using the pure compound. Likewise, chlorogenic acid showed a positive significant correlation with TEAC method. Based on these results it could be found differences in nutraceuticals properties between tomatoes cultivars, and also identify bioactive compound which contribute to these properties.