In vitro compatibility of Pimpinella anisum and Origanum vulgare essential oils with nematophagous fungi and their effects against Nacobbus aberrans

SOSA, ANA LAURA; GIRARDI, NATALIA SOLEDAD; ROSSO, LAURA CRISTINA; SALUSSO, FABRICIO; ETCHEVERRY, MIRIAM GRACIELA; PASSONE, MARÍA ALEJANDRA

JOURNAL OF PEST SCIENCE

SPRINGER HEIDELBERG

Año: 2020

1612-4758

The soil nematode, N. aberrans (Thorne & Allen), is one of the recurrent biotic adversities in undercover crops, whose presence in the horticultural area of Río Cuarto, Argentina, is reported from the ?80s to the present day. The use of botanical extracts in combination with biological control agents could be a promising method for the control of this nematode. Nematicidal effects of pimpinella anisum (anise) and Oreganum vulgare (oregano) essential oils (EOs) against second-stage juveniles (J2) of N. aberrans, were evaluated in vitro. Fisher LSD test showed significant nematicidal (α=0.05) effects of two EOs tested, with LD100 of 200 and 600 μL L-1 for anise and oregano, respectively. A compatibility assay between EOs and five nematophagous fungi were microscopic and macroscopically assayed on soil extract solid medium (SESM) conditioned at different water activities (0.99 and 0.98 aw) and incubated at 30, 25 and20 °C. Purpureocillium lilacinum SR14 was the fungal strains that showed compatibility at levels of spore germination, propagules viability and growth rate under the different anise oil doses, temperatures and aw assayed.As conclusion the integrate application of anise EO with the nematophagous fungus, by combining two action mechanisms, could generate, on the one hand a rapid larval mortality effect, given by the EO and on the other hand, a parasitic effect, that has the advantage of remaining in time under variable environmental conditions.