Voltammetric iodommetric tritiation of ascorbic acid with dead-stop end-point detection in fresh vegetables and fruit samples

VERDINI, RA; LAGIER, CM

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

American Chemical Society

Año: 2000 vol. 48 p. 2812 - 2817

0021-8561

The present work describes a method for determining ascorbic acid, which combines iodometry with a voltammetric technique to detect the end-point of the titration. In addition, the validity of the method applied to natural vegetable or fruit samples was assessed. The results were compared with those obtained by an accurate method such as HPLC using UV detection. Similar values of ascorbic acid for different natural samples were obtained by means of this approach (p > 0.05). The limit of quantification was 0.1 mg. This technique presents the advantage of other electroanalytical methods such as avoiding filtration or ultracentrifugation steps, with the additional benefit of using the platinum electrodes, which are routinely used in the laboratory. These facts allow a rapid and efficient quantification of ascorbic acid with very low cost of reagents and equipment.