Omic sciences for analysis of different Prosopis species

PICARIELLO, G.; SCIAMMARO L. P.; PUPPO M. C.; MAMONE, G.

Prosopis as a Heat Tolerant Nitrogen Fixing Desert Food Legume 1st Edition

ELSEVIER

Lugar: Amsterdam; Año: 2021;

Omic sciences such as metabolomic, lipidomic and proteomic were used for characterizing and identifying chemical compounds from seed germ such as flavonoid, lipids and proteins of different species of Prosopis. Reversed phase-HPLC-diode array detector and nanoflow-HPLC coupled to tandem mass spectrometry (MS/MS) were used. Fatty acids of Prosopis germ were detected by MALDI-TOF mass spectrometry (MS), and phospholipids were isolated by hydroxyapatite chromatography, characterized by MALDI-TOF MS and quantified by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Proteins were also studied by mono- and two-dimensional electrophoretic profiles, ATR-FTIR, essential amino acid content and in vitro digestibility assays.