Effect of galactooligosaccharides:maltodextrin matrices on the recovery of lactobacillus plantarum after spray-drying

NATALIA SOSA; ESTEBAN GERBINO; MARINA A. GOLOWCZYC; CAROLINA SCHEBOR; ANDREA GOMEZ-ZAVAGLIA; E. E. TYMCZYSZYN

FRONTIERS IN MICROBIOLOGY

FRONTIERS RESEARCH FOUNDATION

Año: 2016 vol. 7 p. 584 - 586

1664-302x

In this work maltodextrins were added to commercial galacto-oligosaccharides (GOS) in a 1:1 ratio and their thermophysical characteristics were analyzed. GOS:MD solutions were then used as matrices during spray-drying of L. plantarum CIDCA 83114. The obtained powders were equilibrated at different relative humidities (RH) and stored at 5 and 20ºC for 12 weeks, or at 30ºC for 6 weeks.The Tgs of GOS:MD matrices were about 20-30o 29 C higher than those of GOS at RH within 11 and 52%. A linear relation between the spin-spin relaxation time (T2) and T-Tg parameter was observed for GOS:MD matrices equilibrated at 11, 22, 33 and 44% RH at 5, 20 and 30ºC. Spray-drying of L. plantarum CIDCA 83114 in GOS:MD matrices allowed the recovery of 93% microorganisms. In contrast, only 64% microorganisms were recovered when no MD were included in the dehydration medium. Survival of L. plantarum CIDCA 83114 during storage showed the best performance for bacteria stored at 5o 34 C. In a further step, the slopes of the linear regressions provided information about the rate of microbial inactivation for stored at different temperatures and RH. Using GOS:MD matrices as a dehydration medium enhanced the recovery of L. plantarum CIDCA 83114 after spray-drying. This strategy allowed for the first time the spray-dryingstabilization of a potentially probiotic strain in the presence of GOS.