DETECTION AND QUANTIFICATION OF Aspergillus SECTION Flavi IN STORED PEANUTS BY REAL-TIME PCR OF nor-1 AND EFFECTS OF STORAGE CONDITONS ON AFLATOXINS PRODUCTION

PASSONE, M.A.; ROSSO, L.C.; CIANCIO, A.; ETCHEVERRY, M.G.

Congreso; VI Latin American Congress of Mycotoxicology and II International Symposium on Algal and Fungal Toxins for Industry; 2010

To apply a rapid, sensitive and specific assay, not inhibited by matrix effects, to detect and quantify Aspergillus section Flavi spp. in stored peanuts. The assays were performed in combination with an efficient and economic fungal DNA extraction procedure, tested in peanuts stored and conditioned at different water activities during a 4-month period. The effects of fungal density and storage conditions on aflatoxin produced were also investigated.