Optimization of Enzymatic Degumming Process for Sunflower Oil using Response Surface Methodology: Efficiency and Oil Yield.

LAMAS, D.; CONSTENLA, D.; CECI, L.

RESEARCH JOURNAL OF CHEMISTRY AND ENVIRONMENT

DR JYOTI GARG

Año: 2022 vol. 26

0972-0626

Response surface methodology was used to study theoptimum processing conditions for enzymaticdegumming of sunflower oil. Degumming assays wereperformed using a phospholipase A1 (Lecitase® Ultra)and an acyltransferase (LysoMax® Oil). A 2k-1fractional factorial design was carried out tosimultaneously study the effects of pH, temperature,enzyme concentration, buffer/substrate ratio and timeon the phosphorus content. For the response obtained,a polynomial model was developed through multiplelinear regression analysis. Lecitase® Ultra wasaffected by all studied factors while the buffer/substrateratio did not significantly influence the performance ofLysoMax® Oil.The optimum conditions to achieve the lowestphosphorus content were reached at 50 °C, pH 5 andan enzyme dosage of 200 U/kg of oil during 90 minutesusing Lecitase® Ultra and 59 °C, pH 5.9 and anenzyme dosage of 197 U/kg of oil during 89 minutesusing LysoMax® Oil. Under optimal conditions, thephosphorus content decreased to less than 3 mg/kg withboth enzymes. The oil yield was 96.80 g/100g and 96.85g/100g with the treatment using phospholipase A1 andacyltransferase respectively.