INVESTIGADORES
VINDEROLA Celso Gabriel
artículos
Título:
Effect of a non-lethal High Pressure Homogenization treatment on the in vivo response of probiotic lactobacilli
Autor/es:
TABANELLI, G.; BURNS, P.; PATRIGNANI, F; GARDINI, F.; LANCIOTTI, R.; REINHEIMER, J.; VINDEROLA, G.
Revista:
FOOD MICROBIOLOGY
Editorial:
ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 32 p. 302 - 307
ISSN:
0740-0020
Resumen:
Low levels of High Pressure of Homogenization (HPH)
can be applied directly to lactic acid bacteria cells in order to enhance some functional
properties. In a previous work we observed that a 50 MPa HPH treatment
increased Lactobacillus paracasei A13
hydrophobicity and resistance to simulated gastric digestion. The aim of this
work was to assess the HPH treatment in
vivo effects on probiotic lactobacilli interaction capacity with the gut
and on their ability to induce IgA cell proliferation in mice intestine. BALB/c
mice received FITC-labelled cultures of strains, previously treated or not
(control) at 50 MPa. Fluorescently
labelled cells were studied in the intestine of animals sacrificed 10 and 30
min after intragastric intubation. HPH-treated
and control cultures of each strain were orally administered to mice for 2, 5
or 7 consecutive days. The number of IgA-producing cells in the gut was studied
by immunohistochemistry.
HPH treated probiotic lactobacilli modified their interaction with small
intestine. HPH-treated cells induced a higher IgA response compared to
untreated ones, in a strain- and feeding period-dependent way. HPH treatment
could increase some in vivo
functional characteristics of probiotic strains, highlighting the potential of
this technique in dairy probiotic sector.