Conservación de sangre aviar de matadero, evaluación del uso de distintos aditivos

ZBRUN, M.V.; ALTINA M.G.; BONANSEA, E.F; SIGNORINI, M.L., SOTO, L.P., SEQUEIRA, G.J., ROSMINI, M.R. & FRIZZO, L.S

Rosario, Santa Fe

Congreso; XII congreso y XXX Reunión Anual de la Sociedad de Biología de Rosario; 2010

Sociedad de Biología de Rosario

The use of avian blood seems to be a useful alternative to the simple discarding of this by-product with high biochemical oxygen demand. Additionaly, blood can be used as raw material in both feed and food industries on the basis of its excellent nutritional value and functional properties. The main problem is the recovery and storage of blood from slaughterhouse until to processing. Different biotechnology strategies were developed with the aim to increase the product shelf-life at room temperature. The objective of this study was to compare the effect of different additives (used as biopreservatives) on plasma proteins. Three samples of avian blood from slaughterhouse with anticoagulant were taken and they were transported to the laboratory. Samples were divided into five equal parts for the different treatments: 1) non-incubated control blood (T1), 2) blood stored at refrigeration (4ºC) (T2), 3) blood stored at room temperature (T3), 4) blood + ammonium citrate, beef extract, lactose, Na2HPO3 and NaH2PO3 (T4), and 5) blood + NaCl, beef extract, lactose, Na2HPO3 and NaH2PO3 (T5). T4 and T5 were stored at room temperature for 24 h. Plasma was obtained and the level of proteolysis were analyzed by SDS-PAGE. Differences in the protein degradation were observed according the treatment. T2 showed similar proteolysis than T1. In contrast, T3 and T5 showed a significant protein degradation. Proteolysis was minimal in T4. These data suggest that T4 additives could extend the blood shelf-life at room temperature for, at least, 24h.