IN VITRO SCREENING AND IN VIVO COLONIZATION PILOT MODEL OF LACTOBACILLUS PLANTARUM LP5 AND CAMPYLOBACTER COLI DSPV 458 IN MICE

RUIZ, MARÍA J.; ZBRUN, VIRGINIA; SIGNORINI, MARCELO; ZIMMERMANN, JORGE; SOTO, LORENA; ROSMINI, MARCELO; FRIZZO, LAUREANO

ARCHIVES OF MICROBIOLOGY

SPRINGER

Año: 2021

0302-8933

The objective of this work was to determine the antibacterial effect of Lactobacillus plantarum strains against Campylobacter coli strains and to conduct experimental colonization pilot models in mice for both microorganisms. Inhibition assays allowed to evaluate and select L. plantarum LP5 as the strain with the highest antagonistic activity against C. coli and with the best potential to be used in the in vivo study. Adult 6-week old female Balb/cCmedc mice were lodged in two groups. Treated group (LAB-G) was administered with 9.4 log10CFU/2 times/wk of L. plantarum LP5. Lactobacillus plantarum LP5 was recovered from feces and cecum of inoculated mice. However, when the bacteria stopped being administered, the probiotic counts decreased. Experimental colonization by Campylobacter coli was realized with five groups of mice. All animals were treated with antibiotics in drinking water. Four groups were administered once with different C. coli strains (C. coli DSPV 458: 8.49 log10CFU; C. coli DSPV 567: 8.09 log10CFU; C. coli DSPV 570: 8.46 log10CFU; C. coli DSPV 541: 8.86 log10CFU, respectively). From 8 h, the inoculated mice with different strain of C. coli were colonized because the pathogen were detected in feces. Lactobacillus plantarum LP5 tolerated the gastrointestinal conditions of the murine model without generating adverse effects on animals. Campylobacter coli DSPV 458 colonized the mice without causing infection and lodged in the digestive tract of the animals generating a reproducible colonization model. Both models combined could be used as protection murine models against pathogens to test alternative control tools to antibiotics.