Protective Effect of an Inoculum of Lactic Acid Bacteria from Bovine Origin Against Salmonella Serotype Dublin in the Intestinal Tract of Mice

FRIZZO, L.S. ; ZBRUN, M.V.; SOTO, L.P.; BERTOZZI, E.; SIGNORINI, M.L.; SEQUEIRA, G.J.; MARTI, L.E.; DALLA SANTINA, R.; PERDIGÓN, G.; ROSMINI, M.R.

JOURNAL OF ANIMAL AND VETERINARY ADVANCES

MEDWELL ONLINE

Año: 2010 vol. 9 p. 2113 - 2122

1680-5593

The purpose of this study was to determine the capacity of an inoculum of Lactic Acid Bacteria (LAB) of bovine origin-constituted by Lactobacillus casei DSPV 318T, Lactobacillus salivarius DSPV 315T and Pediococcus acidilactici DSPV 006Tcontaining about 109 CFU dose of each one of the strains to inhibit in vivo the Salmonella serotype Dublin DSPV 595T effects and reduce the pathologic consequences in a conventional group of mice experimentally challenged with a 2.5 x 105 cfu dose of the pathogen bacteria. Before the Salmonella serotype Dublin was administrated, the treated Group (LAB-G = 31 mice) received a 0.1 mL dose of LAB inoculum by daily gavages during 10 days. The control group (C-G = 32 mice) was only inoculated with the pathogen. Individual weight gain, group food consumption and accumulated morbidity and mortality were daily determined. Each animal health was controlled twice daily throughout all the experiment. The lesion caused by the pathogen were visualized by a histopathologic analysis. The protection of animal facing the challenge of the used pathogen was demostrated not only by the highest survival rate of the mice treated by the inoculum versus the control ones but also by the presence  of lactic bacteria inoculated mice that did not get ill throughout the experiment. The lactic acid bacteria treatment protected the mice that had been challenged by Salmonella serotype Dublin DSPV 595T. This effect was demostrated by the higher survival rate of the treated mice when compared to those in the control group.