Inhibitor activity of lactiplantibacillus plantarum LP5 on thermotolerant campylobacter with different biofilm-forming capacities

RUIZ, MARÍA JULIA; SIRINI, NOELÍ ESTEFANÍA; STEGMAYER, MARÍA ÁNGELES; SOTO, LORENA PAOLA; ZBRUN, MARÍA VIRGINIA; OLIVERO, CAROLINA RAQUEL; WERNING, MARÍA LAURA; ACOSTA, FEDERICO FRANCISCO; SIGNORINI, MARCELO LISANDRO; FRIZZO, LAUREANO SEBASTIÁN

Journal of Applied Microbiology

Wiley-Blackwell Publishing Ltd

Año: 2023

AimsTo evaluate the biofilm-forming capacity of thermotolerant Campylobacter (TC) strains from poultry production and to analyse the inhibitory capacity of Lactiplantibacillus plantarum LP5 against TC on different materials.Methods and ResultsBiofilm-forming capacity by Campylobacter jejuni and Campylobacter coli was analysed by cell adhesion in polystyrene plates. TC were classified as non-biofilm-forming (NBF, 1.3%), weak biofilm-forming (WBF, 68.4%), moderate biofilm-forming (MBF, 27.6%), and strong biofilm-forming (SBF, 2.7%). The inhibitory capacity of L. plantarum LP5 against TC was tested on stainless-steel, nylon, aluminium, and glass disks (treated group) and compared with biofilm-forming TC (control group). L. plantarum LP5 was inoculated, and then TC. Biofilm was removed in both experimental groups and TC and LP5 bacterial counts were performed. The L. plantarum LP5 presence reduced the formation of TC biofilm (P < 0.001). The material type and strain category influenced biofilm formation, with stainless-steel and the SBF strain being the material and TC having the highest adhesion (P < 0.001). L. plantarum LP5 formed similar biofilm on all materials (P = 0.823).ConclusionsThis trial showed very promising results; L. plantarum LP5 could be incorporated as a bio-protector of TC on different surfaces.