EVALUACIÓN DE BIOMARCADORES DE ESTRÉS OXIDATIVO EN DIFERENTES POBLACIONES DE ADULTOS DE Cydia pomonella DE LA PATAGONIA ARGENTINA

OLGA L ANGUIANO; ELIZABETH MAERO

Agriscientia

Facultad de Ciencias Agropecuarias Universidad Nacional de Córdoba

Lugar: Córdoba; Año: 2018 vol. 35 p. 35 - 42

Living organisms are exposed to natural environmental pollutants. In this study, antioxidative biomarkers in codling moth Cydia pomonella from different field populations and from a laboratory strain were evaluated. Field populations analyzed were from Neuquén (Centenario), Río Negro (Allen, Gral. Roca1, Gral. Roca2, Gral. Roca3 y Gral. Godoy) y Chubut (Sarmiento1 y Sarmiento2) provinces. Enzymatic antioxidants such as catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) - and non-enzymatic, endogenous glutathione (GSH) levels were evaluated. GST activity found in all field populations insects were significantly lower than laboratory ones. CAT activity in Gral. Roca1 population was statistically different and turned 2.13 times lower than that from the reference strain. No significant differences in SOD activities between different populations were evident. GSH levels observed in Sarmiento2 codling moths was significantly different and resulted 1.57 times higher than the laboratory ones. Except Gral. Godoy, all field populations tested had significantly lower GSH levels compared with the laboratory insects. Alterations in GST and GSH could indirectly reflect the presence of oxidative stress.Key words: codling moth, biomarkers, glutathione S-transferase, catalase, superoxide dismutase, reduced glutathione.