PROBIEN 20416
INSTITUTO DE INVESTIGACION Y DESARROLLO EN INGENIERIA DE PROCESOS, BIOTECNOLOGIA Y ENERGIAS ALTERNATIVAS
Unidad Ejecutora - UE
artículos
Título:
ORGANOPHOSPHATE PESTICIDE ENVIRONMENTAL EXPOSURE: ANALYSIS OF SALIVARY CHOLINESTERASE AND CARBOXILESTERASE ACTIVITIES IN PRESCHOOL CHILDREN AND THEIR MOTHERS
Autor/es:
VANINA BULGARONI, MARÍA GABRIELA ROVEDATTI, GUILLERMO SABINO, GLADIS MAGNARELLI
Revista:
ENVIRONMENTAL MONITORING AND ASSESSMENT
Editorial:
SPRINGER
Referencias:
Año: 2011
ISSN:
0167-6369
Resumen:
A pilot study was conducted to evaluate the usefulness of salivary cholinesterase and carboxylesterase as biomarkers of exposure to environmental organophosphate pesticides. Ninety samples were obtained from women and 62
samples from their
preschool -aged children who live near an agricultural area of the Upper Valley of the Negro River (Patagonia, Argentina) where pesticides are applied six months a year. Each participant donated two samples under similar conditions: one in the pre-exposure period and another during the pulverization period. Demographic information, potential confounders and risk behaviors were registered. Active or passive smoking had no effect on these enzymes activity in either group. During the pulverization period, cholinesterase activity was not detectable in 76% of the childrens samples and 23% of the mothers samples. Comparing samples collected during the pulverization period with respect to the pre-pulverization period, the average mother and child cholinesterase activity decreased by 65.7% (p < 0.001) and 85.8% (p < 0.001), respectively. Also, mother and child carboxylesterase activity decreased by 27.5% (p < 0.001) and 41.9% (p < 0.01), respectively. Child carboxylesterase activity in the pulverization period was associated to the habit of eating dust outdoors (p < 0.01). The most frequent inhibition levels observed for cholinesterase and carboxylesterase activity were between 70-100% and 0-29%, respectively, in both groups studied. This shows that at the same level of exposure, cholinesterase was more sensitive to inhibition than carboxylesterase. Therefore, carboxylesterase might more properly reflect the degree of environmental organophosphate exposure and may have potential as a novel tool for biomonitoring.
