Identification of thermophilic lactic acid bacteria using rep-PCR

DE URRAZA P. J, GÓMEZ ZAVAGLIA A., LOZANO M. E., ROMANOWSKI V., DE ANTONI G. L.

Journal of Dairy Research

Cambridge Journals

Lugar: Cambridge; Año: 2000 vol. 67 p. 381 - 392

0022-0299

DNA fingerprints of lactic acid bacteria were generated by polymerase chain reaction using a primer based on the repetitive elements found in the genome of Streptococcus pneumoniae (BOX-PCR). The method made it possible to identify 37 isolates from raw milk, industrial starters and yogurt. Differentiation at species, subspecies and strain level was possible for Lactobacillus delbrueckii subsp. lactis, Lb. delbrueckii subsp bulgaricus and Str. thermophilus. BOX-PCR was also applied to studying the strain composition of a starter culture and the direct detection of strains in commercial fermented milk.