IMMUNOLOGICAL AND PHYSIOLOGICAL EFFECTS OF Saccharomyces cerevisiae RC016 ALONE AND IN COMBINATION WITH DEOXYNIVALENOL

GARCIA, R.G; DOGI, C; PAYROS, D; GRECO, C; CHULZE, S.N; DE MORENO,A ; OSWALD I; CAVAGLIERI, L.R

Amsterdam

Congreso; congreso; 2016

Probiotics have been explored in order to replace antibiotics as growth promoters in animal feed. They represent a potential safe advance to control enteric bacterial diseases and to improve gut immunity. Saccharomyces cerevisiae RC016 was previously isolated from gut pig and showed in vitro beneficial and mycotoxin adsorbent properties. The aim was to evaluate beneficial properties of Saccharomyces cerevisiae RC016 in weaned piglets and in an ex vivo assays. Secretory IgA (s-IgA) levels, intestinal cytokines, goblet cells and production parameters were evaluated in a pig model. For the in vivo assays, a total of six pigs were weaned at 21 days of age and assigned to two groups: Control Group (CG) and Yeast Group (YG). Animals received yeast strain during three weeks. Feed and water were available ad libitum. Animals were weighed every 3 days. The feed given and the remainder were weighed daily. After 22 days animals were sacrificed by a lethal injection of sodium pentobarbital. Growth parameters determined were: total weight gain (TWG), feed efficiency (FE) and feed conversion rate (FCR). Small intestine was recovered for determination of goblet cells and s-IgA. For the ex vivo assay, jejunal explants were obtained from 5 weeks old crossbred piglets. The treatments were: 1) porcine jejunal explants, 2) porcine jejunal explants exposed for 3 h to 10 μM deoxinivalenol (DON), 3) porcine jejunal explants with 107 UFC/ml yeast strain, 4) porcine jejunal explants pre-incubated 1 h with 107 UFC/ml yeast strain and then exposed for 3 h to 10 μM DON. The explants were incubated at 39°C. For CCL20, IL-1β, IL-8 and IL-22 gene expression, total RNAs were extracted and then reverse transcription and qPCR steps were performed. Oral administration of S. cerevisiae RC016 increased s-IgA, the number of goblet cells in small intestine and all the growth parameters assayed. In the ex vivo model, a toxic effect of DON was observed as an increase of proinflammatory cytokines expression. The presence of the yeast strain showed a strong tendency to counteract this toxic effect. In conclusion S. cerevisiae RC016 is a promising candidate for feed additives formulation to improve animal growth and gut immune system. This yeast strain could be able to counteract the toxic effect of feed naturally contaminated with DON. The use of additives based on beneficial micro-organisms instead of chemical products is a safer and eco-friendly option to increase animal productivity with a minimum environmental impact.