INVESTIGADORES
CHULZE Sofia Noemi
congresos y reuniones científicas
Título:
Fusarium subglutinans/F. temperatum in Argentinian maize
Autor/es:
FUMERO VERONICA; REYNOSO, M.M.; CHULZE , S.N
Lugar:
Martina Franca
Reunión:
Congreso; ISM MycoRed International Conference Europe 2013. Global Mycotoxin Reduction Strategies,; 2013
Institución organizadora:
ISM
Resumen:
Maize samples collected from two main maize producing areas from Argentina, the Northwest region (NOA) and the central region, distand one from another 1875 Km, were evaluated for isolation of Fusarium subglutinans and Fusarium temperatum. From 1080 strains isolated belonging to the Fusarium genera, 114 were identified morphologically from monosporic cultures as Fusarium subglutinans/Fusarium temperatum, of which 21% were isolated from the central area, while the remaining 79% were isolated from the NOA area. From the 114 strains, DNA was extracted using CTAB method and sequencing of the TEF-1α using both primers (EF-1 and EF-2) was done. The sequences were compared with sequences available in the GenBank and Fusarium data base. One group of strains showed between 99% and 100% of homology with F. temperatum strains from MUCL Collection, Belgium, and the other group, showed 100% homology with F. subglutinans strains from KSU collection, USA. Most of the strains identified as F. temperatum were isolated from maize from the NOA region, and a small number of the strains from this region were identified as F. subglutinans. All the strains isolated from the central region were identified as F. subglutinans. Among the strains isolated from the central region, both mating (MATE-1 and MATE-2) were detected by PCR. Among the F. temperatum identified from NOA, both mating types were also detected (MAT1-1 and MAT1-2). The ability of the strains to produce beauvericin (BEA) and fusaproliferin (FUS) on gamma irradiated maize grains (40% humidity), incubated at 25°C ± 1°C, during 28 days in darkness was evaluated. The analysis and detection of the mycotoxins was done by HPLC-UV. Strains isolated from the two areas were BEA and FUS producers. This is the first report of F. temperatum isolation from maize harvested in Argentina.
