Efficacy of antioxidants mixtures on growth fumonisin production and hydrolitic enzyme production by Fusarium verticillioides and Fusarium proliferatum in vitro on maize based media

MARIA MARTA REYNOSO; TORRES, A.M; RAMIREZ, M.L; RODRIGUEZ, M.I; CHULZE, S; MAGAN, N

MYCOLOGICAL RESEARCH

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2002 vol. 106 p. 1093 - 1099

0953-7562

The effect of single or mixtures of antioxidants on the lag phase prior to growth, growth rate, hydrolytic enzyme production (/V-acetyl-¥â-glucosaminidasc, ¥â -D-glucosidase and ¥á-D-galactosidase) and fumonisin production by Fusarium verticillioides and F. proliferatum was evaluated on maize-based media at 25 ¡ÆC, and under different water activity (aW) conditions. An increase ¢®n the lag phase (h) was observed for both F. verticillioides and F. proliferatum especially with propyl paraben (PP) + butylated hydroxyanisole (BHA) treatments at all aw levels tested. For both spccies PP alone or in combination with BHA, at concentrations of 0.5 and 1 mM reduced the growth rales by >85% at the three aw levels tested (0.995; 0.98 and 0.95). PP +butylated hydroxytoluene (BHT) or trihydroxybutyrophenonc (THBP) were less effective in controlling growth, regardless of aw level. Combinations of PP + BHA reduced the fumonisin concentrations produced by F. verticillioides and F. proliferatum at 0.995 and 0.98 aw significantly. However, at low concentrations of antioxidants (0.5 mM) some slimulation in fumonisin production was observed with some treatments. The efficacy of the treatments was reflected in the impact on enzyme production. In the untreated control the highest total enzyme activity of three hydrolytic enzymes was observed at 0.995 avv after 96 h. All the antioxidant treatments alone or combined resulted in a significant reduction (P < 0.001) of the total enzyme activity at the aw levels tested. Only lOmM THBP produced an increase in the total amount of N-acetyl- ¥â ?D-glucosidase by both F. verticillioides and F. proliferatum. For the three enzymes single factors: time, avv and antioxidant treatments, most two and all three way interactions were significant (P < 0.001). The use of combined mixtures of antioxidants could be a good strategy to diminish the entry of fumonisin into the animal feed and human food chains