Optimization of ultrasound assisted green solvent extraction of polyphenols from mango 'criollo' by-products

OJEDA GONZALO; SGROPPO, SONIA C; SANCHEZ MORENO C; DE ANCOS BEGOÑA

Congreso; 14th World Congress on Polyphenols Applications: Polyphenols Applications 2021; 2021

International Society of Antioxidant in Nutrition and Health

Introduction: Mango (Mangifera indica) ´Criollo´ is found in the northeast region of Argentina. These under commercialized fruit can be exploited as a source of bioactive compounds (1). The objective was to optimize the ultrasound assisted extraction of phenolic compounds from mango ´Criollo´ (MC) using a green solvent (2).Material & Methods: Fully ripped fruit (n=50) were harvested in Corrientes, Argentina. Three products were obtained: MC peel (MCP), MC pulp (MCPu) and MC seed (MCS). A three level-variable central composite design was used (60 experiments). Ultrasound generator equipment was used (500 Watts-20 kHz). Total phenolic content (TPC) and phenolic profile by HPLC-ESI-QTOF-MS/MS were determined.Results: TPC in MCP was maximized with 6.5 minutes, amplitude 60% and ethanol content 46%. The extraction from MCPu required 30 minutes, amplitude 75% and ethanol content 25%. MCS required 20 minutes, amplitude 100% and ethanol content 49%. HPLC analysis indicated that mangiferin was present in the three sections showing higher concentration in MCP. It was remarkable the high concentration of ellagic acid in MCS.Conclusion: Underused mango ´Criollo´ can be employed as a source of powerful antioxidant phenolic compounds by an adequate selection of extraction variables to achieve maximum yield from each section of the fruit.