INVESTIGADORES
ALONSO Silvia Del Valle
artículos
Título:
Interaction of sodium nitroprusside/liposome system with bovine serum albumin: a short-cut determination.
Autor/es:
TAIRA MC, STERN AL, ESCOBAR ME, ADRAGNA N, ALONSO-ROMANOWSKI S.
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
Kluwer Academic Publishers
Referencias:
Lugar: Netherlands; Año: 2003 vol. 25 p. 1425 - 1429
ISSN:
0141-5492
Resumen:
A rapid determination of protein-liposome binding was developed to predict the circulation time of the system within an animal, which is a function of the amount and type of protein bound. The binding pattern of albumin to liposomes, with and without sodium nitroprusside (SNP), was analyzed by SDS-PAGE. Liposomes were made of egg yolk lecithin, soybean lecithin and dimyristoyl lecithin, and contained SNP. They bound 58%, 26% and 100% bovine serum albumin, respectively, when compared to their corresponding controls lacking SNP. The method applied is simpler and significantly faster than ordinary chemical determinations.
