Liposome/DNA systems: correlation between association, hydrophobicity and cell viability

CHIARAMONI, NS, SPERONI, L ,TAIRA, MC, ALONSO S. DEL V.

BIOTECHNOLOGY LETTERS

Springer

Lugar: Netherlands; Año: 2007 vol. 29 p. 1637 - 1644

0141-5492

Abstract Small unilamellar vesicles associated withplasmidDNAshowedmaximumassociation efficiencyfor a cationic mixture of egg phosphatidylcholine(EPC):1,2-dioleoyl-sn-glycero-3-phosphoethanolamine(DOPE):di-1,2-dioleoyl-3-trimethyl ammonium pro-pane (DOTAP) (16:8:1 molar ratio) [65%], followedby neutral lipids EPC:1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE):cholesterol (Chol)(2:2:1 molar ratio) [30%], and a polymerized formula-tion 1,2-bis(10,12-tricosadiynoyl)sn-glycero-3-phos-phocholine (DC8,9PC):DMPE:Chol (2:2:1 molarratio) [11%]. The hydrophobicity factor (HF) for theseformulations followed the trend DC8,9PC:DMPE:CHOL < EPC:DMPE:Chol < EPC:DOPE DOTAP,and DNA association did not alter this trend. Resultssuggest that the higher the HF value, the more fluid themembrane and the higher the efficiency of DNAassociation. On the other hand, no differences wereobserved in cell toxicity with lipids up to 1 mg/ml inVERO cells. Keywords  Cell viability - Hydrophobicity - Liposomes - Plasmid DNA - Polymeric lipid