CONICET | Buscador de Institutos y Recursos Humanos

EXTRACTION, IDENTIFICATION AND REUTILIZATION OF PHYTOSTEROLS FROM INDUSTRIAL WASTE IN TREATMENT OF CITRUS CANKER María J. RODRIGUEZ-VAQUERO1*, Sofía SOSA-MARMOL1 y Fabiana M. SAGUIR1 1 Cátedra de Microbiología General, Facultad de Bioquímica, Química y Farmacia ?Universidad Nacional de Tucumán - CONICET ? Ayacucho 471, Tucumán, Argentina, 4000.* Corresponding author: mariajo@fbqf.unt.edu.ar .Tucuman is responsible of 89.8% of country lemon production and Argentina is the first producer of lemon in the world. One problem in this activity is citrus canker caused by Xanthomonas citri subsp. citri. The vinification process generates a great amount of residues. The possibility of reutilization of these wastes is a new alternative for the final disposal of the waste. The aim of this work was the recovery of phytosterols (PS) present in winery wastes, such as grape skin and grape seeds; the development of techniques for FS extraction, investigation of the effect of extracted PS from winery wastes on the viability of X. citri sp citri in vitro and in vivo assays. Results showed that the applied extraction method for PS was effective to remove the phytosterol fraction of wastes. The compounds present in these fractions were studied using TLC and were confirmed and cuantified with GC-mass and the results demonstrate the presence of B-sitosterol, stigmasterol and stigmatanol in two winery wastes studied. The antimicrobial assays showed that extracted PS of grape skin and grape seeds and commercial PS were effective to inhibit the growth of X. citri in laboratory media and in vivo. Ours results demonstrated that the use of winery waste, as source of PS is a promising environmentally friendlier method to control the presence of X. citri in citrus.Xanthomonas citri subsp citri; citrus canker; phytosterols

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