SYNERGISTIC EFFECT OF TWO BACTERIOCINS PRODUCED BY LACTOBACILLI STRAINS ON Listeria spp. GROWTH

GARCÍA MARÍA JOSÉ; ASURMENDI, PAULA; RUIZ, FRANCISCO; PASCUAL, LILIANA; BARBERIS, LUCILA

Estancia Grande, San Luis

Otro; XXXII Reunión científica anual de la Sociedad de Biología de Cuyo; 2014

Sociedad de Biología de Cuyo

In recent years there is a growing interest in the combination of different antimicrobial substances to enhance theinhibitory effect on pathogen microorganisms.Bacteriocins L23 and L60 produced by Lactobacillus fermentum L23and Lactobacillus rhamnosus L60, respectively, have previously demonstrated the ability to inhibit the growth ofseveral pathogen microorganisms including Listeria spp. isolated from food samples. The aim of this study was toevaluate the interactions between bacteriocins L23 and L60 to inhibit Listeriaspp growth. Minimum inhibitoryconcentration (MIC) of each bacteriocin alone and combined were evaluated by the broth microdilution method andthe checkerboard assay, respectively, on L. monocytogenes, L. innocua and L. welshimerigrowth. MIC values wereused to calculate the fractional inhibitory concentration (FIC) and results were interpreted as follows: synergy,FIC≤1; indifference, 1