Evaluation of the antioxidant activity of natural polyphenols extracted from fibre microparticles of sweet cherries (prunus avium L.)

ARAMBURU AGOSTINA; BRUNO NICOLÁS; ANDREA E. MANJARRES RAMOS; FELDENBLUM MAIA; SZEW CAMILA; ROJAS ANA M.; BASANTA FLORENCIA; ERLEJMAN ALEJANDRA

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Sweet cherries discarded by harvest can be a useful source of bioactive compounds, recovering valuable compounds such as polyphenols. Natural polyphenols were co-extracted in fiber microparticles (MPCs) obtained from sweet cherries (Prunus avium L.) discarded at harvest. These compounds may exert cytoprotective actions in the gastrointestinal tract by decreasing oxidative stress. This work aimed to evaluate the antioxidant capacity and cytotoxicity of polyphenols co-extracted in MPCs obtained from sweet cherries. MPCs obtained after saturated steam blanching of cherries, processing with deionized water and freeze-drying, retained polyphenolic compounds: tetrameric proanthocyanidins (631 ± 34 mg/100 g MPCs) and smaller amounts of anthocyanins, flavonoids, and hydroxycinnamates. Undifferentiated (ND: 3d culture) and differentiated (DIF: 21d) Caco-2 cells were used as a model of the intestinal epithelial cells. Cell viability was evaluated through MTT assay. Caco-2 cells were co-incubated 2h with polyphenol extract (8-752 μg polyphenols/mL). The range of concentrations tested did not present cytotoxicity. The antioxidant capacity of polyphenols was evaluated by dichlorofluorescence (DCF) assay. Oxidative stress was induced with tert-butyl-hydroperoxide 3mM for 1h; cells treated increased oxidation 50%, compared to control (untreated) cells. ND cells were co-incubated with 0.5-10.0 μg/mL of polyphenol extract during oxidation, obtaining 20% antioxidant protection (p