INVESTIGADORES
VIRKEL Guillermo Leon
artículos
Título:
Effect of the ionophore antibiotic monensin on the ruminal biotransformation of benzimidazole anthelmintics
Autor/es:
VIRKEL, G., LIFSCHITZ, A., SALLOVITZ, J., INZA, G., LANUSSE, C.
Revista:
The Veterinary Journal
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2004 vol. 167 p. 265 - 271
ISSN:
1090-0233
Resumen:
The benzimidazole (BZD) anthelmintics, netobimin (NTB) pro-drug and
albendazole sulphoxide (ABZSO) are reduced to albendazole (ABZ) by
ruminal microflora. The aim of the current work was to evaluate the
influence of the ionophore monensin (MON) on the in vitro
biotransformation of NTB and ABZSO by sheep ruminal fluid. Ruminal
fluid, collected from Corriedale sheep, was preincubated (24 h) either
without (control) or with known MON concentrations (0.5, 1.5 and 3.0
microg/mL) at 38 degrees C under a CO2 atmosphere. Afterwards, aliquots
from both MON-pretreated and control ruminal fluid samples were
incubated (30 and 60 min) with 2 microg/mL of either NTB or ABZSO.
Incubated samples were chemically extracted and analysed by High
Performance Liquid Chromatography to quantify the metabolites formed.
The rate of ABZ production after 30 min of NTB incubation with control
ruminal fluid was 0.023 microg/min. Conversely, the rates of ABZ
formation were significantly (P<0.05) lower (0.009, 0.011 and 0.013
microg/min) when NTB was incubated with ruminal fluid pretreated with
MON (at 0.5, 1.5 and 3.0 microg/mL, respectively). After both incubation
periods, the reduction of ABZSO to ABZ was 22 to 70% lower when the
ruminal fluid was preincubated with the different MON concentrations.
The lower ABZ production observed in the presence of MON may result in a
modified availability of this molecule in the gastrointestinal (GI)
tract and hence, on its anthelmintic efficacy against GI nematodes.