Effect of enzymes on the oil solvent extraction from sunflower collets with different moisture content.?

PALLA C; RODRIGUEZ L.M.; FERNÁNDEZ M. B.; PEREZ E. E.; CRAPISTE G. H.

Gante

Congreso; 14th Euro Fed Lipids Congress Fats, Oils and Lipids: Innovative Approaches towards a Sustainable Future.; 2016

European Federation for the Science and Technology of Lipids

Enzyme-assisted solvent oil extraction from sunflower collets (porous cylinders obtained from pressed sunflower cake by expanding) at 12 and 18% d.b. (dry basis) moisture content was studied by means of laboratory tests carried out in a stirred batch extractor at 50 °C. Five commercial enzymes with different activities ALPHALASETMNP®, LAMINEX®, FOODPRO®Viscothin gc, VISCOZYME L® was used. These conditions were: enzyme-to-meal ratio ratio 2% (w/w); meal:solvent ratio 1:10 (Kg/L); pH, natural seed and stirring speed of 200 rpm (NRe =11,355); extraction time, 2 h. The enzyme was diluted in distilled water and sprayed onto the solid sample, thus the moisture content in each sample was adjusted to the desired level (12% and 18% d.b.). The efficiency of the treatment was measured in terms of the oil yield compared with the control samples. Fiber wall structure collapsed by the enzymatic action was analyzed by neutral detergent fiber (NDF) and microscopic images. Tocopherols content was measured as quality of oil by HPLC.LSD Fisher test showed, with an error of less than 5%, that after enzimatic treatment there were no significant differences (p=0.5058) in mean values of oil but they varied because of final moisture content (p=0.006). These results were corroborated by the images and NDF values. Enzyme treatment was more effective in the tocopherol extraction from samples with minor moisture content. Although the application of enzymes in sunflower collets did not improve the oil yield extraction, higher tocopherol content was obtained with different profiles according to the enzyme employed.