Enzymatic Digestion Coupled to Surfactant-Assisted Dispersive Liquid-Liquid Microextraction: AMild Approach for Determining Polybrominated Diphenyl Ethers in Human Hair Sample

MAMMANA, SABRINA; ABRAHAM, EMILIA; CAMARGO, ALEJANDRA; ALVARO VAZQUEZ; JORGELINA C. ALTAMIRANO

Chemistry Select

Wiley-Blackwell

Lugar: United Kingdom; Año: 2020 vol. 5 p. 2179 - 2184

nzymatic digestion, coupled to surfactant assisted-dispersive liquid-liquid microextraction (ED-SA-DLLME), is proposed as a mild innovative sample preparation methodology to determine polybrominated diphenyl ethers (PBDEs) in human hair samples by gas chromatography. The sodium dodecyl sulfate as apromoting agent in the ED stage also acts as adispersive agent in DLLME, providing an advantageous synergy between sample preparation stages. Experimental variables of the ED-SA-DLLME were optimized using a multivariate method. The optimal experimental conditions were: proteinase K 0.96 U mL-1, SDS 1% (w/v), 37 °C, and 60 min for ED; and 50 μL tetrachloroethylene for SA-DLLME. The notable analytical figures of merit showed LOQ in the range of 30?430 pg g- 1, relative standard deviations < 9%, and recoveries of 90?94%. The PBDEs were found in human hair samples < 0.07 ng g-1.