Anti-inflammatory, antioxidant, antihypertensive, and antiarrhythmic effect of indole-3-carbinol, a phytochemical derived from cruciferous vegetables

PRADO, NATALIA JORGELINA; RAMIREZ, DANIELA; MAZZEI, LUCIANA; PARRA, MICAELA; CASAROTTO, MARIANA; CALVO, JUAN PABLO; CUELLO CARRIÓN, DARÍO; PONCE ZUMINO, AMIRA ZULMA; DIEZ, EMILIANO RAÚL; CAMARGO, ALEJANDRA; MANUCHA, WALTER

Heliyon

Elsevier Ltd

Lugar: London; Año: 2022 vol. 8

2405-8440

Cardiovascular inflammation and oxidative stress are determining factors in high bloodpressure and arrhythmias. Indole-3-carbinol is a cruciferous-derived phytochemicalwith potential anti-inflammatory and antioxidant effects. However, its implications onthe modulation of cardiovascular inflammatory-oxidative markers are unknown.Objectives: To establish the effects of indole-3-carbinol on the oxidative-inflammatoryproarrhythmic conditions associated with hypertension. Materials: Histological,biochemical, molecular, and functional aspects were evaluated in 1) Culture of mouseBV-2 glial cells subjected to oxidative-inflammatory damage by lipopolysaccharides(100 ng/mL) in the presence or absence of 40 μM indole-3-carbinol (n=5); 2) Malespontaneously hypertensive rats (SHR) and Wistar Kyoto rats receiving indole-3-carbinol (2000 ppm/day, orally) during the first 8 weeks of life (n=15); 3) Isolated rathearts were submitted to 10 min regional ischemia and 10 min reperfusion. Results: 1)lipopolysaccharides induced oxidative stress and increased inflammatory markers;indole-3-carbinol reversed both conditions (interleukin 6, tumor necrosis factor α, theactivity of nicotinamide adenine dinucleotide phosphate oxidase, nitric oxide, induciblenitric oxide synthase, heat shock protein 70, all p