INVESTIGADORES
MEINI Maria Rocio
artículos
Título:
Metallo-beta-lactamases withstand low Zn(II) conditions by tuning metal-ligand interactions
Autor/es:
JAVIER M. GONZÁLEZ; MARÍA ROCÍO MEINI; PABLO E. TOMATIS; FRANCISCO J. MEDRANO MARTÍN; JULIA A. CRICCO; ALEJANDRO J. VILA
Revista:
NATURE CHEMICAL BIOLOGY
Editorial:
NATURE PUBLISHING GROUP
Referencias:
Lugar: Londres; Año: 2012 vol. 8 p. 698 - 700
ISSN:
1552-4450
Resumen:
A number of multiresistant bacterial pathogens inactivate antibiotics by producing Zn(II)-dependent beta-lactamases. We show that metal uptake leading to an active dinuclear enzyme in the periplasmic space of Gram-negative bacteria is ensured by a cysteine residue, an unusual metal ligand in oxidizing environments. Kinetic, structural and affinity data show that such Zn(II)-cysteine interaction is an adaptive trait that tunes the metal binding affinity, thus enabling antibiotic resistance at restrictive Zn(II) concentrations.#La primer autoría de este trabajo es compartida
