Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity

TRUCCO, V.; REINHEIMER, J.; QUIBERONI, A.; SUÁREZ, V.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: London; Año: 2011 vol. 110 p. 935 - 942

1364-5072

Aims: The aim of this work was to study the adsorption step of two new temperate bacteriophages (Cb1/204 and Cb1/342) of Lactobacillus delbrueckii and to isolate phage-resistant derivatives with interesting technological properties. Methods and Results: The effect of divalent cations, pH, temperature and cell viability on adsorption step were analyzed. The Ca2+ presence was necessary for the phage Cb1/342 but not for the phage Cb1/204. Both phages showed to be stable at pH values between 3 and 8. Their adsorption rates decreased considerably at pH 8 but remained high at acid pH values. The optimum temperatures for the adsorption step were between 30ºC and 40ºC. For the phage Cb1/342, non-viable cells adsorbed a lower quantity of phage particles in comparison with the viable ones, a fact that could be linked to disorganization of phage receptor sites and/or to the physiological cellular state. The isolation of phage-resistant derivatives with good technological properties from the sensitive strains and their relationship with the cell heterogeneity of the strains were also made. Conclusions: Characterization of the adsorption step for the first temperate Lact. delbrueckii phages isolated in Argentina was made and phage-resistant derivatives of their host strains were obtained.Significance and impact of study: Some phage-resistant derivatives isolated exhibited good technological properties with the prospective to be used at industrial level.