PERSONAL DE APOYO
VEGGETTI Mariela Iris
artículos
Título:
Differential effect of serum from bipolar versus schizophrenic patients on spontaneous acetylcholine release at mammalian neuromuscular junction
Autor/es:
M. VEGGETTI, S DE LORENZO, A. LOSAVIO AND S. MUCHNIK
Revista:
CLINICAL NEUROPHYSIOLOGY
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2006 vol. 117 p. 2645 - 2652
ISSN:
1388-2457
Resumen:
Objective:
The diagnosis of bipolar
disease frequently requires a long time since the age of onset, especially because
the disease is misdiagnosed with schizophrenia. The aim of the present work was
to investigate whether sera from bipolar patients have an active substance that
allows making a fast identification of the disease.
Methods:
Sera from healthy
volunteers, euthymic and non-stabilized bipolar patients, and schizophrenic
patients were passively transferred into CF1 mice and after 2 day injections,
MEPP frequency from diaphragm muscles was recorded. The same procedure was performed
with sera fraction of high and low MW (cut-off 3000).
Results:
Sera from non-stabilized
bipolar patients induced a decreased MEPP frequency and occluded the
presynaptic inhibitory effect of the specific adenosine A1 receptor
agonist 2-chloro-N6-cyclopentyl-adenosine (CCPA) in the recipient mice, while
in the euthymic bipolar group spontaneous secretion reached control values
although the action of CCPA was still prevented. Similar results were obtained
with low MW sera fraction from euthymic and non-stabilized bipolar patients.
The addition of adenosine deaminase to the sera fraction prevented the
modification of spontaneous ACh release. In mice injected with sera from
schizophrenic patients, MEPP frequency was within control values and CCPA induced
its typical inhibitory action.
Conclusions:
These results indicate that
bipolar patients contain in their blood an active substance compatible with
adenosine, which was able to modify spontaneous ACh release in the recipient
mice. This effect was not observed with sera from healthy volunteers and schizophrenic
patients. The increase of adenosine concentration may result from synaptic
hyperactivity that presumably plays a role in the symptoms of bipolar disorder
and/or may derive from peripheral cells through a more general mechanism.
Significance:
The different results
obtained with bipolar and schizophrenic sera raise the possibility that the
passive transfer model could be used as a diagnostic test in the future